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vesicular stomatitis virus g vsv g envelope expressing plasmid pmd2 g  (Addgene inc)


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    Structured Review

    Addgene inc vesicular stomatitis virus g vsv g envelope expressing plasmid pmd2 g
    Vesicular Stomatitis Virus G Vsv G Envelope Expressing Plasmid Pmd2 G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 15580 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vesicular+stomatitis+virus/psPAX2+(Plasmid+%2312260)/pm41856116-689-32-41
    Average 98 stars, based on 15580 article reviews
    vesicular stomatitis virus g vsv g envelope expressing plasmid pmd2 g - by Bioz Stars, 2026-10
    98/100 stars

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    Produced:

    Article Title: FAM222A, Part of the BET-Regulated Basal Endothelial Transcriptome, Is a Novel Determinant of Endothelial Biology and Angiogenesis—Brief Report
    Article Snippet: For FAM222A overexpression studies in HUVEC, the cDNA of full-length FAM222A was cloned into the lentiviral EGFP vector pULTRA (24129, Addgene) by digestion of the restriction enzyme EcoRI. .. Lentiviral vectors were produced by triple transient transfection of human embryonic kidney 293T cells with a packaging plasmid psPAX2 (12260, Addgene), a plasmid encoding the envelope of vesicular stomatitis virus (VSVg; pMD.2G; 12259, Addgene) and the pULTRA plasmid encoding FAM222A or GFP (green fluorescent protein) using lipofectamine 3000 per manufacturer’s protocol. .. Viruses were collected 48 and 72 hours after transfection, tittered, and incubated with HUVEC for 16 hours in presence of 8 μg/mL polybrene (TR-1003-G, Sigma) at a range of multiplicity of infection.

    Article Title: Chemokine receptor distribution on the surface of repolarizing T cells.
    Article Snippet: T cells migrate constitutively with a polarized morphology, underpinned by signaling compartmentalization and discrete cytoskeletal organizations, giving rise to a dynamic and expansive leading edge, distinct from the stable and constricted uropod at the rear.. In vivo, the motion and function of T cells at various stages of differentiation is highly directed by chemokine gradients.. When cognate ligands bind chemokine receptors on their surface, T cells respond by reorientating their polarity axis and migrating toward the source of the chemokine signal.

    Transfection:

    Article Title: FAM222A, Part of the BET-Regulated Basal Endothelial Transcriptome, Is a Novel Determinant of Endothelial Biology and Angiogenesis—Brief Report
    Article Snippet: For FAM222A overexpression studies in HUVEC, the cDNA of full-length FAM222A was cloned into the lentiviral EGFP vector pULTRA (24129, Addgene) by digestion of the restriction enzyme EcoRI. .. Lentiviral vectors were produced by triple transient transfection of human embryonic kidney 293T cells with a packaging plasmid psPAX2 (12260, Addgene), a plasmid encoding the envelope of vesicular stomatitis virus (VSVg; pMD.2G; 12259, Addgene) and the pULTRA plasmid encoding FAM222A or GFP (green fluorescent protein) using lipofectamine 3000 per manufacturer’s protocol. .. Viruses were collected 48 and 72 hours after transfection, tittered, and incubated with HUVEC for 16 hours in presence of 8 μg/mL polybrene (TR-1003-G, Sigma) at a range of multiplicity of infection.

    Article Title: Nanoencapsulation of etoposide promotes increased long-term DNA damage, greater induction of senescence and reduces population regrowth of lung cancer cells
    Article Snippet: Lung cancer is the most common type of cancer and the leading cause of cancer-related deaths worldwide.. The nanoencapsulation of etoposide, a topoisomerase II inhibitor and widely used chemotherapeutic drug, may overcome the limitations related to low aqueous solubility and non-specific toxicity, in addition to enabling a sustained release of etoposide and promoting long-term effects.. Here we developed etoposide-loaded lipid-core nanocapsules (ETO NC) having unimodal particle size (~150 nm), drug content of 0.5 mg mL 1 and encapsulation efficiency of ~99 %.

    Article Title: Chemokine receptor distribution on the surface of repolarizing T cells.
    Article Snippet: T cells migrate constitutively with a polarized morphology, underpinned by signaling compartmentalization and discrete cytoskeletal organizations, giving rise to a dynamic and expansive leading edge, distinct from the stable and constricted uropod at the rear.. In vivo, the motion and function of T cells at various stages of differentiation is highly directed by chemokine gradients.. When cognate ligands bind chemokine receptors on their surface, T cells respond by reorientating their polarity axis and migrating toward the source of the chemokine signal.

    Plasmid Preparation:

    Article Title: FAM222A, Part of the BET-Regulated Basal Endothelial Transcriptome, Is a Novel Determinant of Endothelial Biology and Angiogenesis—Brief Report
    Article Snippet: For FAM222A overexpression studies in HUVEC, the cDNA of full-length FAM222A was cloned into the lentiviral EGFP vector pULTRA (24129, Addgene) by digestion of the restriction enzyme EcoRI. .. Lentiviral vectors were produced by triple transient transfection of human embryonic kidney 293T cells with a packaging plasmid psPAX2 (12260, Addgene), a plasmid encoding the envelope of vesicular stomatitis virus (VSVg; pMD.2G; 12259, Addgene) and the pULTRA plasmid encoding FAM222A or GFP (green fluorescent protein) using lipofectamine 3000 per manufacturer’s protocol. .. Viruses were collected 48 and 72 hours after transfection, tittered, and incubated with HUVEC for 16 hours in presence of 8 μg/mL polybrene (TR-1003-G, Sigma) at a range of multiplicity of infection.

    Article Title: Synthetic host defense peptide inhibits SARS-CoV-2 replication in vitro .
    Article Snippet: .. Plasmid pMD2.G, encoding the G envelope protein from Vesicular stomatitis virus (VSV-g, a gift from Didier Trono, plasmid #12259, Addgene, Watertown, MA), was used as a positive control. .. Cell culture media was changed 24 h after transfection, and the cell-free supernatant was collected at 48 h post-transfection, filtered through a 0.45-μm cellulose acetate filter (Ahlstrom Munksjo, Helsinki, Finland), and 1 mL aliquots were stored at −80°C until further use.

    Article Title: Engineering T cell resistance to HIV-1 infection via knock-in of peptides from the heptad repeat 2 domain of gp41
    Article Snippet: Plasmids for the expression of wild type spCas9 alone or in combination with gRNA were pcDNA3.3-hCas9 (Addgene #41815) and pX330-T2A-BFP (Addgene # 64216), respectively. .. To generate lentiviral particles, 8 × 10 5 293 T cells in a 6 cm dish were cotransfected with 2 μg of HIV-1 packaging plasmid pCMVΔ8.2 R (Addgene # 12263); 0.5 μg of the pCMV-VSV-G plasmid (Addgene # 8454) expressing protein G from vesicular stomatitis virus (VSV-G); and 3 μg of one of the pUCHR transfer vector constructed as described in previous paragraph. .. Transfection was performed for 6 h using Lipofectamine 2000 reagent (Thermo Scientific, USA) according to the manufacturer’s instruction, then the culture medium was replaced.

    Article Title: Synthetic host defense peptide inhibits SARS-CoV-2 replication in vitro
    Article Snippet: De Francesco, plasmid# 155297, Addgene, Watertown, MA), a series of codon-optimized spike glycoproteins from different betacoronaviruses were synthesized and cloned using 5’ Not I and 3’ Xba I into a modified pcDNA3.1 vector in frame with an N-terminal DYKDDDDK peptide to generate a comprehensive panel of pseudotyped viral particles containing the spike protein from SARS-CoV-2 B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), B.1.617.2 (Delta), B.1.429 (Epsilon), B.1.617.1 (Kappa), C.37 (Lambda), B.1.621 (Mu), B.1.525 (Eta), B.1.526 (Theta), and B.1.1.529 (Omicron BA.1) variants, as well as SARS-CoV and MERS-CoV. .. Plasmid pMD2.G, encoding the G envelope protein from Vesicular stomatitis virus (VSV-g, a gift from Didier Trono, plasmid #12259, Addgene, Watertown, MA), was used as a positive control. .. Cell culture media was changed 24 h after transfection, and the cell-free supernatant was collected at 48 h post-transfection, filtered through a 0.45-μm cellulose acetate filter (Ahlstrom Munksjo, Helsinki, Finland), and 1 mL aliquots were stored at −80°C until further use.

    Article Title: Nanoencapsulation of etoposide promotes increased long-term DNA damage, greater induction of senescence and reduces population regrowth of lung cancer cells
    Article Snippet: Lung cancer is the most common type of cancer and the leading cause of cancer-related deaths worldwide.. The nanoencapsulation of etoposide, a topoisomerase II inhibitor and widely used chemotherapeutic drug, may overcome the limitations related to low aqueous solubility and non-specific toxicity, in addition to enabling a sustained release of etoposide and promoting long-term effects.. Here we developed etoposide-loaded lipid-core nanocapsules (ETO NC) having unimodal particle size (~150 nm), drug content of 0.5 mg mL 1 and encapsulation efficiency of ~99 %.

    Article Title: Engineering T-Cell Resistance to HIV-1 Infection via Knock-In of Peptides from the Heptad Repeat 2 Domain of gp41
    Article Snippet: Plasmids for the expression of wild-type spCas9 alone or in combination with gRNA were pcDNA3.3-hCas9 (Addgene number 41815) and pX330-T2A-BFP (Addgene number 64216), respectively. .. To generate lentiviral particles, 8 × 10 5 293 T cells in a 6-cm dish were cotransfected with 2 μg of HIV-1 packaging plasmid pCMVΔ8.2 R (Addgene number 12263); 0.5 μg of the pCMV-VSV-G plasmid (Addgene number 8454) expressing protein G from vesicular stomatitis virus (VSV-G); and 3 μg of one of the pUCHR transfer vector constructed as described in the previous paragraph. .. Transfection was performed for 6 h using Lipofectamine 2000 reagent (Thermo Scientific, USA) according to the manufacturer’s instructions, and then the culture medium was replaced.

    Article Title: Chemokine receptor distribution on the surface of repolarizing T cells.
    Article Snippet: T cells migrate constitutively with a polarized morphology, underpinned by signaling compartmentalization and discrete cytoskeletal organizations, giving rise to a dynamic and expansive leading edge, distinct from the stable and constricted uropod at the rear.. In vivo, the motion and function of T cells at various stages of differentiation is highly directed by chemokine gradients.. When cognate ligands bind chemokine receptors on their surface, T cells respond by reorientating their polarity axis and migrating toward the source of the chemokine signal.

    Virus:

    Article Title: FAM222A, Part of the BET-Regulated Basal Endothelial Transcriptome, Is a Novel Determinant of Endothelial Biology and Angiogenesis—Brief Report
    Article Snippet: For FAM222A overexpression studies in HUVEC, the cDNA of full-length FAM222A was cloned into the lentiviral EGFP vector pULTRA (24129, Addgene) by digestion of the restriction enzyme EcoRI. .. Lentiviral vectors were produced by triple transient transfection of human embryonic kidney 293T cells with a packaging plasmid psPAX2 (12260, Addgene), a plasmid encoding the envelope of vesicular stomatitis virus (VSVg; pMD.2G; 12259, Addgene) and the pULTRA plasmid encoding FAM222A or GFP (green fluorescent protein) using lipofectamine 3000 per manufacturer’s protocol. .. Viruses were collected 48 and 72 hours after transfection, tittered, and incubated with HUVEC for 16 hours in presence of 8 μg/mL polybrene (TR-1003-G, Sigma) at a range of multiplicity of infection.

    Article Title: Synthetic host defense peptide inhibits SARS-CoV-2 replication in vitro .
    Article Snippet: .. Plasmid pMD2.G, encoding the G envelope protein from Vesicular stomatitis virus (VSV-g, a gift from Didier Trono, plasmid #12259, Addgene, Watertown, MA), was used as a positive control. .. Cell culture media was changed 24 h after transfection, and the cell-free supernatant was collected at 48 h post-transfection, filtered through a 0.45-μm cellulose acetate filter (Ahlstrom Munksjo, Helsinki, Finland), and 1 mL aliquots were stored at −80°C until further use.

    Article Title: Engineering T cell resistance to HIV-1 infection via knock-in of peptides from the heptad repeat 2 domain of gp41
    Article Snippet: Plasmids for the expression of wild type spCas9 alone or in combination with gRNA were pcDNA3.3-hCas9 (Addgene #41815) and pX330-T2A-BFP (Addgene # 64216), respectively. .. To generate lentiviral particles, 8 × 10 5 293 T cells in a 6 cm dish were cotransfected with 2 μg of HIV-1 packaging plasmid pCMVΔ8.2 R (Addgene # 12263); 0.5 μg of the pCMV-VSV-G plasmid (Addgene # 8454) expressing protein G from vesicular stomatitis virus (VSV-G); and 3 μg of one of the pUCHR transfer vector constructed as described in previous paragraph. .. Transfection was performed for 6 h using Lipofectamine 2000 reagent (Thermo Scientific, USA) according to the manufacturer’s instruction, then the culture medium was replaced.

    Article Title: Synthetic host defense peptide inhibits SARS-CoV-2 replication in vitro
    Article Snippet: De Francesco, plasmid# 155297, Addgene, Watertown, MA), a series of codon-optimized spike glycoproteins from different betacoronaviruses were synthesized and cloned using 5’ Not I and 3’ Xba I into a modified pcDNA3.1 vector in frame with an N-terminal DYKDDDDK peptide to generate a comprehensive panel of pseudotyped viral particles containing the spike protein from SARS-CoV-2 B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), B.1.617.2 (Delta), B.1.429 (Epsilon), B.1.617.1 (Kappa), C.37 (Lambda), B.1.621 (Mu), B.1.525 (Eta), B.1.526 (Theta), and B.1.1.529 (Omicron BA.1) variants, as well as SARS-CoV and MERS-CoV. .. Plasmid pMD2.G, encoding the G envelope protein from Vesicular stomatitis virus (VSV-g, a gift from Didier Trono, plasmid #12259, Addgene, Watertown, MA), was used as a positive control. .. Cell culture media was changed 24 h after transfection, and the cell-free supernatant was collected at 48 h post-transfection, filtered through a 0.45-μm cellulose acetate filter (Ahlstrom Munksjo, Helsinki, Finland), and 1 mL aliquots were stored at −80°C until further use.

    Article Title: Nanoencapsulation of etoposide promotes increased long-term DNA damage, greater induction of senescence and reduces population regrowth of lung cancer cells
    Article Snippet: Lung cancer is the most common type of cancer and the leading cause of cancer-related deaths worldwide.. The nanoencapsulation of etoposide, a topoisomerase II inhibitor and widely used chemotherapeutic drug, may overcome the limitations related to low aqueous solubility and non-specific toxicity, in addition to enabling a sustained release of etoposide and promoting long-term effects.. Here we developed etoposide-loaded lipid-core nanocapsules (ETO NC) having unimodal particle size (~150 nm), drug content of 0.5 mg mL 1 and encapsulation efficiency of ~99 %.

    Article Title: Imaging and quantitative analysis of integrin-dependent cell-matrix adhesions
    Article Snippet: pRC-CMV-REV1B , encoding the post-transcriptional regulator Rev1b necessary for efficient synthesis of viral proteins , Addgene , Cat#164443. .. pHDMG-G VSV ENV , coding for the envelope protein of Vesicular Stomatitis Virus , Addgene , Cat#164440. .. pHDM-TAT 1B , encoding the Tat1b protein, which facilitates viral entry into target cells , Addgene , Cat#164442.

    Article Title: Engineering T-Cell Resistance to HIV-1 Infection via Knock-In of Peptides from the Heptad Repeat 2 Domain of gp41
    Article Snippet: Plasmids for the expression of wild-type spCas9 alone or in combination with gRNA were pcDNA3.3-hCas9 (Addgene number 41815) and pX330-T2A-BFP (Addgene number 64216), respectively. .. To generate lentiviral particles, 8 × 10 5 293 T cells in a 6-cm dish were cotransfected with 2 μg of HIV-1 packaging plasmid pCMVΔ8.2 R (Addgene number 12263); 0.5 μg of the pCMV-VSV-G plasmid (Addgene number 8454) expressing protein G from vesicular stomatitis virus (VSV-G); and 3 μg of one of the pUCHR transfer vector constructed as described in the previous paragraph. .. Transfection was performed for 6 h using Lipofectamine 2000 reagent (Thermo Scientific, USA) according to the manufacturer’s instructions, and then the culture medium was replaced.

    Article Title: Chemokine receptor distribution on the surface of repolarizing T cells.
    Article Snippet: T cells migrate constitutively with a polarized morphology, underpinned by signaling compartmentalization and discrete cytoskeletal organizations, giving rise to a dynamic and expansive leading edge, distinct from the stable and constricted uropod at the rear.. In vivo, the motion and function of T cells at various stages of differentiation is highly directed by chemokine gradients.. When cognate ligands bind chemokine receptors on their surface, T cells respond by reorientating their polarity axis and migrating toward the source of the chemokine signal.

    Positive Control:

    Article Title: Synthetic host defense peptide inhibits SARS-CoV-2 replication in vitro .
    Article Snippet: .. Plasmid pMD2.G, encoding the G envelope protein from Vesicular stomatitis virus (VSV-g, a gift from Didier Trono, plasmid #12259, Addgene, Watertown, MA), was used as a positive control. .. Cell culture media was changed 24 h after transfection, and the cell-free supernatant was collected at 48 h post-transfection, filtered through a 0.45-μm cellulose acetate filter (Ahlstrom Munksjo, Helsinki, Finland), and 1 mL aliquots were stored at −80°C until further use.

    Article Title: Synthetic host defense peptide inhibits SARS-CoV-2 replication in vitro
    Article Snippet: De Francesco, plasmid# 155297, Addgene, Watertown, MA), a series of codon-optimized spike glycoproteins from different betacoronaviruses were synthesized and cloned using 5’ Not I and 3’ Xba I into a modified pcDNA3.1 vector in frame with an N-terminal DYKDDDDK peptide to generate a comprehensive panel of pseudotyped viral particles containing the spike protein from SARS-CoV-2 B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), B.1.617.2 (Delta), B.1.429 (Epsilon), B.1.617.1 (Kappa), C.37 (Lambda), B.1.621 (Mu), B.1.525 (Eta), B.1.526 (Theta), and B.1.1.529 (Omicron BA.1) variants, as well as SARS-CoV and MERS-CoV. .. Plasmid pMD2.G, encoding the G envelope protein from Vesicular stomatitis virus (VSV-g, a gift from Didier Trono, plasmid #12259, Addgene, Watertown, MA), was used as a positive control. .. Cell culture media was changed 24 h after transfection, and the cell-free supernatant was collected at 48 h post-transfection, filtered through a 0.45-μm cellulose acetate filter (Ahlstrom Munksjo, Helsinki, Finland), and 1 mL aliquots were stored at −80°C until further use.

    Expressing:

    Article Title: Engineering T cell resistance to HIV-1 infection via knock-in of peptides from the heptad repeat 2 domain of gp41
    Article Snippet: Plasmids for the expression of wild type spCas9 alone or in combination with gRNA were pcDNA3.3-hCas9 (Addgene #41815) and pX330-T2A-BFP (Addgene # 64216), respectively. .. To generate lentiviral particles, 8 × 10 5 293 T cells in a 6 cm dish were cotransfected with 2 μg of HIV-1 packaging plasmid pCMVΔ8.2 R (Addgene # 12263); 0.5 μg of the pCMV-VSV-G plasmid (Addgene # 8454) expressing protein G from vesicular stomatitis virus (VSV-G); and 3 μg of one of the pUCHR transfer vector constructed as described in previous paragraph. .. Transfection was performed for 6 h using Lipofectamine 2000 reagent (Thermo Scientific, USA) according to the manufacturer’s instruction, then the culture medium was replaced.

    Article Title: Engineering T-Cell Resistance to HIV-1 Infection via Knock-In of Peptides from the Heptad Repeat 2 Domain of gp41
    Article Snippet: Plasmids for the expression of wild-type spCas9 alone or in combination with gRNA were pcDNA3.3-hCas9 (Addgene number 41815) and pX330-T2A-BFP (Addgene number 64216), respectively. .. To generate lentiviral particles, 8 × 10 5 293 T cells in a 6-cm dish were cotransfected with 2 μg of HIV-1 packaging plasmid pCMVΔ8.2 R (Addgene number 12263); 0.5 μg of the pCMV-VSV-G plasmid (Addgene number 8454) expressing protein G from vesicular stomatitis virus (VSV-G); and 3 μg of one of the pUCHR transfer vector constructed as described in the previous paragraph. .. Transfection was performed for 6 h using Lipofectamine 2000 reagent (Thermo Scientific, USA) according to the manufacturer’s instructions, and then the culture medium was replaced.

    Construct:

    Article Title: Engineering T cell resistance to HIV-1 infection via knock-in of peptides from the heptad repeat 2 domain of gp41
    Article Snippet: Plasmids for the expression of wild type spCas9 alone or in combination with gRNA were pcDNA3.3-hCas9 (Addgene #41815) and pX330-T2A-BFP (Addgene # 64216), respectively. .. To generate lentiviral particles, 8 × 10 5 293 T cells in a 6 cm dish were cotransfected with 2 μg of HIV-1 packaging plasmid pCMVΔ8.2 R (Addgene # 12263); 0.5 μg of the pCMV-VSV-G plasmid (Addgene # 8454) expressing protein G from vesicular stomatitis virus (VSV-G); and 3 μg of one of the pUCHR transfer vector constructed as described in previous paragraph. .. Transfection was performed for 6 h using Lipofectamine 2000 reagent (Thermo Scientific, USA) according to the manufacturer’s instruction, then the culture medium was replaced.

    Article Title: Engineering T-Cell Resistance to HIV-1 Infection via Knock-In of Peptides from the Heptad Repeat 2 Domain of gp41
    Article Snippet: Plasmids for the expression of wild-type spCas9 alone or in combination with gRNA were pcDNA3.3-hCas9 (Addgene number 41815) and pX330-T2A-BFP (Addgene number 64216), respectively. .. To generate lentiviral particles, 8 × 10 5 293 T cells in a 6-cm dish were cotransfected with 2 μg of HIV-1 packaging plasmid pCMVΔ8.2 R (Addgene number 12263); 0.5 μg of the pCMV-VSV-G plasmid (Addgene number 8454) expressing protein G from vesicular stomatitis virus (VSV-G); and 3 μg of one of the pUCHR transfer vector constructed as described in the previous paragraph. .. Transfection was performed for 6 h using Lipofectamine 2000 reagent (Thermo Scientific, USA) according to the manufacturer’s instructions, and then the culture medium was replaced.

    Polymer:

    Article Title: Nanoencapsulation of etoposide promotes increased long-term DNA damage, greater induction of senescence and reduces population regrowth of lung cancer cells
    Article Snippet: Lung cancer is the most common type of cancer and the leading cause of cancer-related deaths worldwide.. The nanoencapsulation of etoposide, a topoisomerase II inhibitor and widely used chemotherapeutic drug, may overcome the limitations related to low aqueous solubility and non-specific toxicity, in addition to enabling a sustained release of etoposide and promoting long-term effects.. Here we developed etoposide-loaded lipid-core nanocapsules (ETO NC) having unimodal particle size (~150 nm), drug content of 0.5 mg mL 1 and encapsulation efficiency of ~99 %.

    Transduction:

    Article Title: Chemokine receptor distribution on the surface of repolarizing T cells.
    Article Snippet: T cells migrate constitutively with a polarized morphology, underpinned by signaling compartmentalization and discrete cytoskeletal organizations, giving rise to a dynamic and expansive leading edge, distinct from the stable and constricted uropod at the rear.. In vivo, the motion and function of T cells at various stages of differentiation is highly directed by chemokine gradients.. When cognate ligands bind chemokine receptors on their surface, T cells respond by reorientating their polarity axis and migrating toward the source of the chemokine signal.

    Molecular Weight:

    Article Title: Chemokine receptor distribution on the surface of repolarizing T cells.
    Article Snippet: T cells migrate constitutively with a polarized morphology, underpinned by signaling compartmentalization and discrete cytoskeletal organizations, giving rise to a dynamic and expansive leading edge, distinct from the stable and constricted uropod at the rear.. In vivo, the motion and function of T cells at various stages of differentiation is highly directed by chemokine gradients.. When cognate ligands bind chemokine receptors on their surface, T cells respond by reorientating their polarity axis and migrating toward the source of the chemokine signal.



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